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Highly Immunogenic Trimethyl Chitosan-based Delivery System for Intranasal Lipopeptide Vaccines against Group A Streptococcus

[ Vol. 14 , Issue. 5 ]

Author(s):

Nirmal Marasini, Khairunnisa Abdul Ghaffar, Ashwini Kumar Giddam, Michael R. Batzloff, Michael F. Good, Mariusz Skwarczynski and Istvan Toth   Pages 701 - 708 ( 8 )

Abstract:


Background: Group A streptococcus (GAS) primarily colonizes the mucosal region of the upper respiratory tract, slowly leading to systemic infections. Thus, GAS-specific antibody responses are desirable at mucosal sites for early prevention against GAS colonization.

Methods: Herein, we developed a potent nanoliposomes-based delivery system for mucosally active lipid core peptide (LCP)-based vaccines.

Results: Trimethyl chitosan (TMC)-coated liposomes that bore a B-cell epitope derived from GAS Mprotein, stimulated potent epitope-specific mucosal and systemic antibody titres after only one boost following intranasal immunization in Swiss outbred mice. The immune responses were durable even at day 139 post-primary immunization.

Conclusion: The enhanced vaccine efficacy, lowered dose, and simple and cost-effective process of producing the coated nanoliposomes should be particularly useful in developing potent peptide-based vaccines to prevent infections at the mucosal sites.

Keywords:

Chitosan, group A streptococcus, lipopeptides, liposomes, nasal delivery, peptide vaccines.

Affiliation:

The University of Queensland, School of Chemistry & Molecular Biosciences, St Lucia, QLD, 4072, The University of Queensland, School of Chemistry & Molecular Biosciences, St Lucia, QLD, 4072, The University of Queensland, School of Chemistry & Molecular Biosciences, St Lucia, QLD, 4072, Griffith University, Institute for Glycomics, Gold Coast, QLD, 4222, Griffith University, Institute for Glycomics, Gold Coast, QLD, 4222, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072

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