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Formulation and Optimization of Candesartan Cilexetil Nano Lipid Carrier: In Vitro and In Vivo Evaluation

[ Vol. 14 , Issue. 7 ]

Author(s):

Anjan Paudel, Ameeduzzafar, Syed Sarim Imam, Mohd Fazil, Shahroz Khan, Abdul Hafeez, Farhan Jalees Ahmad and Asgar Ali*   Pages 1005 - 1015 ( 11 )

Abstract:


Purpose: The objective of this study was to formulate and optimize Candesartan Cilexetil (CC) loaded nanostructured lipid carriers (NLCs) for enhanced oral bioavailability.

Method: Glycerol monostearate (GMS), Oleic acid, Tween 80 and Span 40 were selected as a solid lipid, liquid lipid, surfactant and co- surfactant, respectively. The CC-NLCs were prepared by hot emulsion probe sonication technique and optimized using experimental design approach. The formulated CC-NLCs were evaluated for various physicochemical parameters and further optimized formulation (CC-NLC-Opt) was assessed for in vivo pharmacokinetic and pharmacodynamic activity.

Results: The optimized formulation (CC-NLC-Opt) showed particle size (183.5±5.89nm), PDI (0.228±0.13), zeta potential (-28.2±0.99mV), and entrapment efficiency (88.9±3.69%). The comparative in vitro release study revealed that CC-NLC-Opt showed significantly better (p<0.05) release and enhanced permeation as compared to CC-suspension. The in vivo pharmacokinetic study gave many folds increase in oral bioavailability than CC suspension, which was further confirmed by antihypertensive activity in a murine model.

Conclusion: Thus, the results of ex vivo permeation, pharmacokinetic study and pharmacodynamics study suggest the potential of CC-NLCs for improved oral delivery.

Keywords:

Antihypertensive activity, bioavailability study, formulation design, candesartan cilexetil, lipid formulation, nanoparticle.

Affiliation:

Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, Department of Pharmaceutics, College of Pharmacy, Aljouf University, Sakaka, Aljouf, Department of Pharmaceutics, Glocal School of Pharmacy, The Glocal University, Sahararnpur, Uttar Pradesh, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062

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