Submit Manuscript  

Article Details


Gold Nanoparticles Conjugated Levofloxacin: For Improved Antibacterial Activity Over Levofloxacin Alone

[ Vol. 14 , Issue. 8 ]

Author(s):

Paramdeep Bagga, Hifazat Hussain Siddiqui, Juber Akhtar, Tariq Mahmood, Manaal Zahera and Mohd Sajid Khan*   Pages 1114 - 1119 ( 6 )

Abstract:


Background: Levofloxacin is a potent antibiotic with severe side effects due to its high doses. Bacterial resistance may be due to frequent use of antibiotics. Biogenic gold nanoparticles conjugated levofloxacin (Au-HSA-LvN-NPs) were developed by Human Serum Albumin (HSA) and nitrate reductasemediated pathways.

Methods: Au-HSA-LvN-NPs (size = 27.2 ± 1 nm) were readily generated with high emulsion stability zeta potential (-13.3 mV). The developed nanoparticles were also characterized by UVvisible spectroscopy, Transmission Electron Microscopy and Dynamic Light Scattering techniques.

Results: The optimized nanoparticles were found efficient against both Gram-positive bacteria and Gramnegative bacteria specifically S. aureus (MIC-0.373 µg/ml), E. coli (MIC-0.149 µg/ml) and P. aeruginosa (MIC-0.346 µg/ml) respectively.

Conclusion: The efficiency of bioconjugated levofloxacin got improved by 1.94 times, 2.89 times and 1.46 times against S. aureus, E. coli and P. aeruginosa respectively, in comparison to pure levofloxacin.

Keywords:

Gold nanoparticles, human serum albumin, levofloxacin, nitrate reductase, zeta potential, antibiotics.

Affiliation:

Faculty of Pharmacy, Integral University, Lucknow 226026, Faculty of Pharmacy, Integral University, Lucknow 226026, Faculty of Pharmacy, Integral University, Lucknow 226026, Faculty of Pharmacy, Integral University, Lucknow 226026, Department of Biosciences, Integral University, Lucknow 226026, Department of Biosciences, Integral University, Lucknow 226026

Graphical Abstract:



Read Full-Text article