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Continuous Vector-free Gene Transfer with a Novel Microfluidic Chip and Nanoneedle Array

[ Vol. 16 , Issue. 2 ]

Author(s):

Dong Huang, Deyao Zhao, Jinhui Li, Yuting Wu, Lili Du, Xin-Hua Xia, Xiaoqiong Li, Yulin Deng, Zhihong Li and Yuanyu Huang*   Pages 164 - 170 ( 7 )

Abstract:


Background: Delivery of foreign cargoes into cells is of great value for bioengineering research and therapeutic applications.

Objective: In this study, we proposed and established a carrier-free gene delivery platform utilizing staggered herringbone channel and silicon nanoneedle array, to achieve high-throughput in vitro gene transfection.

Methods: With this microchip, fluidic micro vortices could be induced by the staggered-herringboneshaped grooves within the channel, which increased the contact frequency of the cells with the channel substrate. Transient disruptions on the cell membrane were well established by the nanoneedle array on the substrate.

Result: Compared to the conventional nanoneedle-based delivery system, proposed microfluidic chip achieved flow-through treatment with high gene transfection efficiency (higher than 20%) and ideal cell viability (higher than 95%).

Conclusion: It provides a continuous processing environment that can satisfy the transfection requirement of large amounts of biological molecules, showing high potential and promising prospect for both basic research and clinical application.

Keywords:

Drug delivery, gene therapy, chaotic microfluidics, nanoneedle array, staggered herringbone channel, microfluidic chip.

Affiliation:

Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotheranotics, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081, Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotheranotics, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081, Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotheranotics, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081



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