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In vivo Antiplasmodial Activity of Curcumin-Loaded Nanostructured Lipid Carriers

[ Vol. 16 , Issue. 10 ]

Author(s):

Hamid Rashidzadeh, Mahsa Salimi, Somayeh Sadighian*, Kobra Rostamizadeh and Ali Ramazani*   Pages 923 - 930 ( 8 )

Abstract:


Background: It has been shown that curcumin (Cur) has anti-plasmodial activity; however, its weak bioavailability, rapid metabolism, and limited chemical stability have restricted its application in clinical usages. Nanostructured lipid carriers (NLCs) are a type of Drug-Delivery Systems (DDSs) whose core matrix is composed of both solid and liquid lipids.

Objective: The aim of the current study was to prepare and characterize curcumin-loaded nanostructured lipid carriers (Cur-NLC) for malaria treatment.

Methods: For producing NLC, coconut oil and cetyl palmitate were selected as a liquid and solid lipid, respectively. In order to prepare the Cur-NLC, the microemulsion method was applied. General toxicity assay on Artemia salina as well as hemocompatibility was investigated. Anti-plasmodial activity was studied on mice infected with Plasmodium berghei.

Results: The NLCs mean particle size and Polydispersity Index (PI) were 145 nm and 0.3, respectively. Further, the zeta potential of the Cur-NLC was −25 mV. The NLCs indicated a pseudo-spherical shape observed via transmission electron microscopy (TEM). The loading capacity and encapsulation efficacy of the obtained Cur-NLC were 3.1 ± 0.015% and 74 ± 3.32%, respectively. In vitro, Cur release profiles showed a sustained-release pattern up to 5 days in the synthesized Cur-NLC. The results of in vivo antiplasmodial activity against P. berghei revealed that antimalarial activity of Cur-NLC was significantly higher compared with that of free Cur at the dose of 40 mg/kg/day.

Conclusion: The results of this study suggested that NLC would be used as a potential nanocarrier for the treatment of malaria.

Keywords:

Nanostructured lipid carriers (NLCs), Curcumin, Malaria, Plasmodium berghei, Drug-Delivery Systems (DDSs), Polydispersity Index (PI).

Affiliation:

Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan



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