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Delivery Efficacy Differences of Intravenous and Intraperitoneal Injection of Exosomes: Perspectives from Tracking Dye Labeled and MiRNA Encapsulated Exosomes

Author(s):

Xueying Zhou, Zhelong Li, Wenqi Sun, Guodong Yang, Changyang Xing and Lijun Yuan*   Pages 1 - 9 ( 9 )

Abstract:


Exosomes are cell-derived nanovesicles that play vital roles in intercellular communication. Recently, exosomes are recognized as the promising drug delivery vehicles. Up to now, how the in vivo distribution of exosomes is affected by different administration routes has not been fully understood. In the present study, in vivo distribution of exosomes following intravenous and intraperitoneal injection approaches were systemically analyzed by tracking the fluorescence labeled exosomes and qPCR analysis of C. elegans specific miRNA abundance delivered by exosomes in different organs. The results showed that exosomes administered through tail vein were mostly taken up by liver, spleen and lungs while exosomes injected intraperitoneally were more dispersedly distributed. Besides the liver, spleen, and lungs, intraperitoneal injection effectively delivered exosomes into the visceral adipose tissue, making it a promising strategy for obesity therapy. Moreover, the results from fluorescence tracking and qPCR were slightly different, which could be explained by systemic errors. Together, our study reveals that different administration routes cause significant differential in vivo distribution of exosomes, suggesting that optimization of the delivery route is prerequisite to obtain rational delivery efficiency in detailed organs.

Keywords:

Exosomes, in vivo delivery, administration route, efficiency, intraperitoneal injection, intravenous injection

Affiliation:

Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi’an, Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi’an, Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi’an, The State Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi’an, Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi’an



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