Submit Manuscript  

Article Details


Design and synthesis of IMR-23 an oxime derived from nitroimidazole as immunomodulatory molecule

Author(s):

Esmeralda Sánchez-Pavón, Aracely López-Monteon, Delia Hernández-Romero, María de la Soledad Lagunes-Castro, Dxinegueela Yolanda Zanatta-García and Angel Ramos-Ligonio*  

Abstract:


Background. Adjuvants have been obtained empirically by trial and error experiments and today there is a tendency to rational design of adjuvants candidates, which will increasingly achieve effective and safe products. The aim of this work was to design and evaluate the compound IMR-23 derived from nitroimidazole as an immunomodulatory molecule.

Material and Methods. The IMR-23 molecule was obtained by a condensation reaction, cytotoxicity was tested by the sulforhodamine B assay. Adjuvanticity was evaluated in vivo and in vitro in J774A.1 cells and in the mouse model, respectively.

Results. IMR-23 did not show cytotoxicity on HeLa, Vero cells and macrophages J774A.1, was able to induce the production of molecules involved in the inflammatory process, such as, cytokines and chemokines determined by ELISA, to induce the production of antibodies and to generate antigen-specific cells to ovalbumin and against the antigen GST-L1b.

Conclusions. These results open the possibility of further studies to obtain a proper balance of immunogenicity-toxicity in the use of IMR-23 as an adjuvant molecule.

Keywords:

Oxime, Nitroimidazole, Adjuvant, Inflammation, Immune Response, Antibody

Affiliation:

LADISER Química Orgánica, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, LADISER Química Orgánica, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz



Full Text Inquiry