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Topical Melatonin Niosome Gel for the Treatment of 5-FU-Induced Oral Mucositis in Mice

[ Vol. 18 , Issue. 2 ]


Prangtip Uthaiwat, Jureerut Daduang*, Aroonsri Priprem, Chatri Settasatian, Sirinart Chio-Srichan, Yao-Chang Lee, Pramote Mahakunakorn and Patcharee Boonsiri   Pages 199 - 211 ( 13 )


Background: Oral mucositis, one of the most common complications of 5-fluorouracil (5-FU) treatment, leads to several problems, including pain, diarrhea and malnutrition, and reduces the quality of life and subsequent treatments. Melatonin, a neurohormone with anti-inflammatory and antioxidant activities, was encapsulated in niosomes and embedded in a mucoadhesive gel formulation as a Melatonin Niosome Gel (MNG) to perform oral mucositis treatment.

Objective: This study aimed to investigate the effectiveness of MNG for the treatment of 5-FU-induced oral mucositis in mice.

Methods: Oral mucositis was induced in ICR mice by 5-FU and randomly assigned to receive daily applications of the topical oral MNG, a fluocinolone acetonide gel, a blank niosome gel, or no treatment for 5 days in comparison with a normal group. Average body weights, food consumption, and behaviors of the mice as well as microscopic histopathology, Fourier-Transform Infrared Spectroscopy (FTIR) analysis, proinflammatory cytokine levels, and oxidative stress markers of the tongues were monitored and collected after sacrifice.

Results: In comparison to the normal group, the average body weights of the 5-FU-MNG mice did not deviate from that of the normal group, nor was there a significant difference in the time to sleep or licking (p>0.05 for both parameters). In addition, the mice treated with MNG and fluocinolone acetonide did not show significantly different histopathological, FTIR, interleukin-1β or malondialdehyde (MDA) results in the tongues used as the oral tissue samples.

Conclusion: Topical MNG potentially inhibits inflammation and lipid oxidative stress in 5-FU-induced oral mucositis.


Niosomes, melatonin, 5-fluorouracil, mucositis, anti-inflammation, antioxidant, Malondialdehyde (MDA).


Graduate School, Khon Kaen University, Khon Kaen, Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Melatonin Research Group, Khon Kaen University, Khon Kaen, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, National Synchrotron Radiation Research Center, Hsinchu, Taiwan, Department of Pharmaceutical Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen

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