Submit Manuscript  

Article Details


Potential Application of A Synthetic Organo-funtionalized High Load Expandable Mica as A Drug Carrier for Controlled Release

[ Vol. 18 , Issue. 5 ]

Author(s):

M. Mar Orta*, Sara Maisanaba, Santiago Medina-Carrasco and Angeles Jos   Pages 645 - 653 ( 9 )

Abstract:


Background: In this work the cytotoxicity and gastric and gastrointestinal resistance of a high-load synthetic expandable mica, Na-mica-4, is studied for the first time. The hydrophilic character of this clay mineral can be modified by ion exchange reaction between Na+ inorganic cations housed in the interlayer space, and surfactant molecules, resulting in the formation of an organophilic material. This adsorption capability of organic compounds makes them very useful for a wide range of applications, such as their use as drug carriers. Previous studies have shown the high adsorption capacity of organofunctionalized Na-mica-4 of different types of drugs.

Objetive: To carry out initial trials aimed at testing the cytotoxicity of a synthetic organofunctional expandable mica and evaluating its resistance to gastric and gastrointestinal digestion.

Methods: A highly charged sodium mica (Na-mica-4) was synthesized and organofunctional by cationic exchange with an alkylamine, primary amine of 18 carbon atoms (C18-mica-4). Both were characterized by X-ray diffraction, field transmission electron microscopy, surface-specific analysis, differential scanning calorimetry, and thermal gravimetric analysis. In addition, screening cytotoxicity trials were conducted on the human intestinal cell line Caco-2 with C18-mica-4 (0-125 μg/ml).

Results: Only one of the endpoints evaluated (the reduction of tetrazolium MTS salt by dehydrogenase enzymes) showed a significant decrease in cellular viability after 48h at the highest concentration tested. C18-mica-4 shows structural resistance to both, gastric and gastrointestinal, digestion.

Conclusion: A successful development of a functionalized mica has been made with a promising potential application as a carrier to the drug.

Keywords:

High-charge swelling mica, organo-functionalization, cytotoxicity, drug carrier, characterization, synthetic mica.

Affiliation:

Analytical Chemistry Department, Faculty of Pharmacy, University of Sevilla, Profesor García Gonzalez no. 2, Sevilla 41012, Area of Toxicology, University Pablo de Olavide, Carretera de Utrera, Km 1, Sevilla 41013, X-Ray Laboratory (CITIUS), University of Sevilla, Sevilla 41012, Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor García González no. 2, Sevilla 41012

Graphical Abstract:



Read Full-Text article