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Chlorambucil-Chitosan Nano-conjugate: An Efficient Agent Against Breast Cancer Targeted Therapy

Author(s):

Azadeh Shayegh, Farinaz Khalatbari, Niloofar Zonoubi, Farjad Zarazvand, Fatemeh Monavvari, Hadi Hejazinia, Seyed Esmaeil Sadat Ebrahimi, Morteza Pirali Hamedani, Vahid Ali, Shahin Hadadian, Jafar Farzaneh, Mohammad Seyedhamzeh and Mehdi Shafiee Ardestani*   Pages 1 - 8 ( 8 )

Abstract:


Background: Discovering new chemotherapy drugs and techniques with the least side effects is one of the most important and challenging world issues in recent years. Chlorambucil is an anticancer drug that is still commonly used as a primary treatmentin at treatment of some cancers, but it can cause side effects.

Objective: In this study, we decided to use chitosan as a carrier for enhance the uptake of chlorambucil and reduce the toxicity of this drug.

Method: After producing this nanoconjugate compound and analysing its structure by FTIR, DLS and AFM analysis, we investigated the therapeutic and biological effects of this nanoconjugate compound on the MCF-7 cell line (breast cancer).

Results: The results of the MTT assay showed that this nanoconjugate compound not only retained its anti-cancer effect against chlorambucil but also showed less abnormal toxicity. Also, In vitro cellular uptake by flow cytometry indicated the better uptake final product into the MCF-7 cells. The detection of apoptosis induced cell death was confirmed by RT-PCR.

Conclusion: This study has created a prospective pathway for targeting cancer cells using chitosan.

Keywords:

Chlorambucil, breast cancer, chitosan, targeting, Nano-conjugate, treatment.

Affiliation:

Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Faculty of Basic Sciences, Ashrafi Esfahani, Islamic Azad University Central Tehran Branch, Tehran, Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Faculty of Pharmacy, Mashahd University of Medical Sciences, Mashhad, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Chemistry, Faculty of Science, Islamic Azad University, Rasht, Pilot Nanobiotechnology Dept, Pasteur Institute of Iran, Tehran, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran



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