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Development and Biocompatibility Analysis of NBD Peptide Sustained-release Microsphere Scaffold Nanoparticle SP-Sr-CaS/NBD

Author(s):

Xue Li, Lei Wang, Changbing Wang, Caixia Tan, Xifaofang Liu and Yongzhan Zhu*   Pages 1 - 13 ( 13 )

Abstract:


In clinical treatment, it is difficult to carry out effective bone tissue transplantation and anti-inflammatory treat-ment at the same time due to bone defects and osteomyelitis where the tissue is contaminated or infected. As a downstream target of TNF-α, NF-κB has an inhibition effect on the proliferation and differentiation of cells surrounding the lesion. As a negative effect, it leads to slowing of bone growth and development. In this study, the small molecule NBD polypeptide and bone conduction matrix Sr-CaS are microsphere-formed to prepare Sr-CaS, NBD drug-loaded sustained-release micro-spheres, in order to achieve a Sr-CaS/NBD peptide drug-loaded sustained release microsphere scaffold material (SP-Sr-CaS/NBD). We prepared the microspheres and optimized the production process to obtain particles with stable morphologi-cal properties and sustained release properties. In vitro experiments demonstrated that SP-Sr-CaS/NBD can reduce TNF-α-induced cell growth inhibition, caspase-3 activity and NF-κB transcriptional activation as the function of continuous NBD peptide dosing regimen. Also, the introduction of Sr-CaS matrix potentiate microspheres to promote cell proliferation and provides a basis to become a hopeful used 3D bone scaffold material in the future.

Keywords:

NBD, PLGA, Calcium sulfate, Osteomyelitis, Microsphere, Scaffold materials.

Affiliation:

18th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, Department of Orthopaedics & Traumatology, Nanfang Hospital, Southern Medical University, 510515, 15th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, 18th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, 8th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, 8th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000



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