M. Mar Orta*, Sara Maisanaba, Santiago Medina-Carrasco and Angeles Jos Pages 1 - 9 ( 9 )
Background: In this work the cytotoxicity and gastric and gastrointestinal resistance of a high-load synthetic expandable mica, Na-mica-4, is studied for the first time. The hydrophilic character of this clay mineral can be modified by ion exchange reaction between Na+ inorganic cations housed in the interlayer space, and surfactant molecules, resulting in the formation of an organophilic material. This adsorption capability of organic compounds makes them very useful for a wide range of applications, such as their use as drug carriers. Previous studies have shown the high adsorption capacity of organofunctionalized Na-mica-4 of different types of drugs.
Objetive: To carry out initial trials aimed at testing the cytotoxicity of a synthetic organofunctional expandable mica and evaluating its resistance to gastric and gastrointestinal digestion.
Methods: A highly charged sodium mica (Na-mica-4) was synthesized and organofunctional by cationic exchange with an alkilamine, primary amine of 18 carbon atoms (C18-mica-4). Both were characterized by X-ray diffraction, field transmission electron microscopy, surface-specific analysis, differential scanning calorimetry, and thermal gravimetric analysis. In addition, screening cytotoxicity trials were conducted on the human intestinal cell line Caco-2 with C18-mica-4 (0-125 µg/ml).
Results: Only one of the endpoints evaluated (the reduction of tetrazolium MTS salt by dehydrogenase enzymes) showed a significant decrease in cellular viability after 48h at the highest concentration tested. C18-mica-4 shows structural resistance to both, gastric and gastrointestinal, digestion.
Conclusion: A successful development of a functionalized mica has been made with a promising potential application as a carrier to the drug.
High-charge swelling mica, organo-functionalization, cytotoxicity, drug carrier, characterization, synthetic mica
Analytical Chemistry Department, Faculty of Pharmacy, University of Sevilla, Profesor García González n° 2, 41012 Sevilla, Area of Toxicology, University Pablo de Olavide, Carretera de Utrera, Km 1, 41013 Sevilla, XRay Laboratory (CITIUS), University of Sevilla, 41012 Sevilla, Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor García González n° 2, 41012 Sevilla