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Development and Biocompatibility Analysis of NBD Peptide Sustained- Release Microsphere Scaffold Nanoparticle SP-Sr-CaS/NBD

[ Vol. 18 , Issue. 4 ]

Author(s):

Xue Li, Lei Wang, Changbing Wang, Caixia Tan, Xifaofang Liu and Yongzhan Zhu*   Pages 433 - 445 ( 13 )

Abstract:


Background: In clinical treatment, it is difficult to carry out effective bone tissue transplantation and anti-inflammatory treatment at the same time due to bone defects and osteomyelitis where the tissue is contaminated or infected. As a downstream target of TNF-α, NF-κB has an inhibition effect on the proliferation and differentiation of cells surrounding the lesion. As a negative effect, it leads to a reduction in bone growth and development.

Methods: In this study, the small molecule NBD polypeptide and bone conduction matrix Sr-CaS are microspheres, formed to prepare Sr-CaS, NBD drug-loaded sustained-release microspheres in order to achieve a Sr-CaS/NBD peptide drug-loaded sustained release microsphere scaffold material (SP-Sr-CaS/NBD). We prepared the microspheres and optimized the production process to obtain particles with stable morphological properties and sustained release properties.

Result: In vitro experiments demonstrated that SP-Sr-CaS/NBD could reduce TNF-α-induced cell growth inhibition, caspase-3 activity and NF-κB transcriptional activation as the function of continuous NBD peptide dosing regimen.

Conclusion: Also, the introduction of the Sr-CaS matrix potentiates microspheres to promote cell proliferation and provides a basis to become a promising 3D bone scaffold material in the future.

Keywords:

NBD, PLGA, calcium sulfate, osteomyelitis, microsphere, scaffold materials.

Affiliation:

8th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, Guangzhou, Department of Orthopaedics & Traumatology, Nanfang Hospital, Southern Medical University, 510515, 15th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, Guangzhou, Radiology Department, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, Guangzhou, 8th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, Guangzhou, 8th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, 528000, Guangzhou

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